Mechanoporation delivered two circular RNA payloads simultaneously, encoding a CD19 CAR and membrane-bound IL-2, directly into T cells within unfractionated whole blood, with no upstream cell isolation or processing. Boosted samples achieved 52+% double-positive expression (CD19 CAR+ and IL-2+) compared to unboosted controls, while red blood cells and platelets remained unaffected. Read more here.
CD19+ B cells isolated from PBMCs are notoriously resistant to lentiviral transduction. By using mechanoporation to pre-deliver a circular RNA encoding a transduction enhancer, lentiviral GFP transduction reached 47% in boosted cells versus 13% in unboosted controls, a 3.6-fold increase. This demonstrates mechanoporation as a complement to existing viral workflows, extending lentiviral delivery to cell types that have historically been difficult to transduce. Read more here.
Four antibodies were delivered simultaneously into live Ramos B cells and primary B cells to detect phosphorylated BTK (p-BTK) using a Lumit luminescence readout, with no cell lysis required. Portal-enabled live-cell detection achieved luminescence comparable to lysate-based assays, with a 7.3-fold signal induction in stimulated cells versus 4.5-fold for lysate, preserving native cellular context throughout. Read more here.
B2M-targeting CRISPR RNPs were delivered into unstimulated primary T cells using a Galaxy-i cartridge integrated into the Nnano Certus Flex liquid handler in 96-well plate format. Across nearly 100 automated dispenses, the system achieved 74% B2M knockout, 80% dextran-positive delivery, and 72% viability at Day 3. Read more here.
Twenty-seven distinct GFP mRNA constructs were screened in a single automated run using Galaxy-i and the Certus Flex platform, with PBMCs dispensed directly into pre-loaded 96-well plates. GFP expression varied substantially across constructs, with the top performer reaching ~700 MFI, enabling rapid ranking of mRNA construct performance in primary immune cells from a single plate run. Read more here.
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