Getting Started: Setting up the gateway and other materials in the biosafety cabinet

Typical control samples: Properly labeling all of your control and collection tubes before prepping your cells

Priming the booster: When opening a new booster, make sure to rinse the cartridge with 100uL of delivery buffer. This helps to wet the chip and prime it before cells pass through

Boosting: Setting up the ‘No Cargo’ as the first boost through the cartridge. Mixing 50uL of the 2x cell solution with 50uL of delivery buffer. Transfer the full 100uL cell solution to the top of the MicroBooster and attach to the Gateway. Press and hold the illuminated button until all of the liquid flows through. After 30s-1min, add recovery media to your boosted sample

Clean up: Once the experiment is complete, discarding of the MicroBooster cartridge and turning off the Gateway. If there are any spills on the Gateway, highlighting how to remove certain parts of the Gateway for a more thorough clean

Culturing of Cell Lines: In this video, we focused on adherent cells - the same rules apply for suspension cells

Thawing of Primary Immune Cells: In this video, we walkthrough the process of thawing primary immune cells - including a treatment with Benzonase and ideal resting conditions before boosting

ACK and Selection: In this video, we discuss the importance of using gentle isolation and selection methods before boosting

Length of Experiment: In this video, we highlight the importance of preparing media, buffers, tubes and cargo before preparing cells to minimize the length of time the cells are outside optimal culturing conditions

Cargo Considerations: In this video, we highlight the importance of including proper endocytosis controls when testing new cargoes. We also cover some important considerations when using temperature-sensitive or unstable cargoes

Optimizing a Workflow: In this video, we review the parameters to consider when setting up an optimization experiment on the Gateway

See the Millibooster in action! Watch as we run a water demo to show how it connects to a Rotea enabling clinical scale mechanoporation.

Portal mechanoporation integrates seamlessly with the nNano Certus Flex dispenser for automated, plate-based intracellular delivery using the Galaxy-i.