High Throughput Intracellular Delivery with Galaxy-i

Name: Galaxy-i
Brand: Portal

Powered by Portal's universal intracellular delivery platform, Galaxy-i enables scalable, automated plate-based workflows for 10x less than the per-well cost of electroporation and other methods.

Three Integration Options

Choose the configuration that matches your throughput needs, from one 96-well plate to hundreds of 384-well plates.

Galaxy-i Single Dispenser

Offers automated dispensing into multiwell plates. Integrates into fluidics of existing robots and mechanoporates cells immediately before dispensing.

Minimizes time between boosting and plating on cargo
Consistent performance across device
Dispense time per plate remains the same
Minimal modifications of existing systems

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Galaxy-i Eight Dispensers

Offers automated dispensing into multiwell plates at higher throughput. Integrates into fluidics of existing robots across all eight channels.

Minimizes time between boosting and plating on cargo
Consistent performance across device
Dispense time per plate remains the same
Minimal modifications of existing systems
8X the throughput

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Galaxy

High-throughput device for mechanoporation on liquid handler deck. Offers maximum flexibility for liquid handlers. Mechanoporates cells and outputs into a trough ready for liquid handler.

No special software or integrations required
2–50ml
1–100M/ml cells
Compatible with both manual workflows and liquid handlers

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Upgrade Your Screening Workflows

High-throughput mechanical delivery

Cargo Flexibility

Screen small molecules, DELs, peptides, oligos, RNA and CRISPR RNPs.

Easy Workflow Integration

Compatible with both manual workflows and liquid handlers

Cell and Buffer Flexibility

Compatible across cell lines, primary immune and stemcells

Cost-Efficient Scale

10x lower cost per-well than alternatives

Unlock Novel Use Cases

Impermeable Hit Identification

Obtain and validate hits in live cells for impermeable early drug candidates

Impermeable Probes

Intracellular delivery of impermeable tagged probes, proteins, and antibodies

Appnote

Primary Cell RNA & RNPs

Gene edit or modulate primary cells for assays

Appnote

Direct to Biology Assays

Intracellular delivery of DEL libraries

Appnote

HT Primary Cell CRISPR Screens

Conduct large library crispr screening

Appnote

Gene Editing

Deletion, correction, integration

Multiplexed Modifications

Simultaneous multi-cargo delivery, transient programming, novel inhibitors

Appnote

Immune Cell Therapies

Immunotherapies, gene correction, cell-based vaccines

Appnote

iPSCs & Regenerative Medicine

Engineered tissues, disease modeling, drug screening in patient cells

Appnote

How Does the HT Delivery Work?

Our Galaxy system works by boosting cells as they transit through the membrane onto plates with pre-plated cargo where they then take up the material.

How It Works

This works because after boosting the pores in the cell membrane remain open for approximately 30 seconds. During this brief window, cells are dispensed directly onto plates with pre-plated cargo, enabling efficient uptake of materials.

Key Advantages

Pre-loaded plates: prepare cargo libraries in standard plates ahead of time
On-demand processing: Add cells and process when ready – no wait time for cargo prep
High-throughput multiplexing: Screen diverse, well-specific payloads in a single run

Critical Timing Window

Cell membrane pores remain open for 5–30 seconds post-boosting, allowing efficient cargo uptake when cells are dispensed onto pre-plated material. This brief window enables high-throughput processing while maintaining cell viability and function.

Platform Validation Data

Arrayed CRISPR Screening in Primary Cells

Demonstrated proof-of-concept for CRISPR screening in primary cells using the Galaxy-i with Certus Flex. This workflow achieved successful B2M RNP delivery to primary T cells in a high-throughput system—enabling functional genomics applications in clinically relevant cell types.

Consistent Delivery Across Plates

Validated consistent delivery performance in HeLa cells across five 96-well plates using diverse cargo types: 3kDa dextran, labeled oligo, and IgG antibody—demonstrating platform reliability across molecular sizes and formats.

Broad Cell Type Compatibility

Demonstrated successful delivery across multiple cell types, including co-cultured HeLa cells and human PBMCs (shown here: dextran, antibody) - critical for drug discovery programs that require validation across diverse cell models.

Complex Cargo Delivery

Achieved siRNA-mediated B2M knockdown in up to 86% of T cells with a strong dose response - demonstrating the platform's capability of delivering complex functional cargos essential for gene editing, RNA regulation studies, and functional genomics screening.